BELASCO LAB
New York University School of Medicine
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Publications
Venzon M, Das R, Luciano DJ, Burnett J, Park HS, Devlin JC, Kool ET, Belasco JG, Hubbard EJA, Cadwell K (2022).
Microbial byproducts determine reproductive fitness of free-living and parasitic nematodes.
Cell Host Microbe 30: 786-797.
Richards J, Belasco JG (2022).
PABLO-QA: A sensitive assay for quantifying monophosphorylated RNA 5′ ends.
STAR Protoc. 3: 101190.
Levenson-Palmer R, Luciano DJ, Vasilyev N, Nuthanakanti A, Serganov A, Belasco JG (2022).
A distinct RNA recognition mechanism governs Np4 decapping by RppH.
Proc. Natl. Acad. Sci. USA 119: e2117318119.
Hui MP, Belasco JG (2021).
Multifaceted impact of a nucleoside monophosphate kinase on 5′-end-dependent mRNA degradation in bacteria.
Nucleic Acids Res. 49: 11038-11049.
Richards J, Belasco JG (2021).
Riboswitch control of bacterial RNA stability.
Mol. Microbiol. 116: 361-365.
Richards J, Belasco JG (2021).
Widespread protection of RNA cleavage sites by a riboswitch aptamer that folds as a compact obstacle to scanning by RNase E.
Mol. Cell 81: 127-138.
Luciano DJ, Belasco JG (2020).
Np4A alarmones function in bacteria as precursors to RNA caps.
Proc. Natl. Acad. Sci. USA 117: 3560-3567.
Luciano DJ, Levenson-Palmer R, Belasco JG (2019).
Stresses that raise Np4A levels induce protective nucleoside tetraphosphate capping of bacterial RNA.
Mol. Cell 75: 957-966.
Richards J, Belasco JG (2019).
Obstacles to scanning by RNase E govern bacterial mRNA lifetimes by hindering access to distal cleavage sites.
Mol. Cell 74: 284-295.
Luciano DJ, Belasco JG (2019).
Analysis of RNA 5' ends: phosphate enumeration and cap characterization.
Methods 155: 3-9.
Gao A, Vasilyev N, Luciano DJ, Levenson-Palmer R, Richards J, Marsiglia WM, Traaseth NJ, Belasco JG, Serganov A (2018).
Structural and kinetic insights into stimulation of RppH-dependent RNA degradation by the metabolic enzyme DapF.
Nucleic Acids Res. 46: 6841-6856.
Luciano DJ, Vasilyev N, Richards J, Serganov A, Belasco JG (2018).
Importance of a diphosphorylated intermediate for RppH-dependent RNA degradation.
RNA Biol. 15: 703-706
Lodato PB, Thuraisamy T, Richards J, Belasco JG (2017).
Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7.
FEMS Microbiol. Lett. 364: fnx131.
Luciano DJ, Vasilyev N, Richards J, Serganov A, Belasco JG (2017).
A novel RNA phosphorylation state enables 5′ end-dependent degradation in Escherichia coli.
Mol. Cell 67: 44-54.
Belasco JG (2017).
Death by translation: ribosome-assisted degradation of mRNA by endonuclease toxins.
FEBS Lett. 591: 1851-1852.
Bischler T, Hsieh PK, Resch M, Liu Q, Tan HS, Foley PL, Hartleib A, Sharma CM, Belasco JG (2017).
Identification of the RNA pyrophosphohydrolase RppH of Helicobacter pylori and global analysis of its RNA targets.
J. Biol. Chem. 292: 1934-1950.
Belasco JG (2017).
Ribonuclease E: chopping knife and sculpting tool.
Mol. Cell 65: 3-4.
Richards J, Belasco JG (2016).
Distinct requirements for 5′-monophosphate-assisted RNA cleavage by Escherichia coli RNase E and RNase G.
J. Biol. Chem. 291: 5038-5048.
Luciano DJ, Belasco JG (2015).
NAD in RNA: unconventional headgear.
Trends Biochem. Sci. 40: 245-247.
Foley PL, Hsieh PK, Luciano DJ, Belasco JG (2015).
Specificity and evolutionary conservation of the Escherichia coli RNA pyrophosphohydrolase RppH.
J. Biol. Chem. 290: 9478-9486.
Belasco JG (2015).
Way to go, RNA.
RNA 21: 565-566.
Schmidt SA, Foley PL, Jeong DH, Rymarquis LA, Doyle F, Tenenbaum SA, Belasco JG, Green PJ (2015).
Identification of SMG6 cleavage sites and a preferred RNA cleavage motif by global analysis of endogenous NMD targets in human cells.
Nucleic Acids Res. 43: 309-323.
Hui MP, Foley PL, Belasco JG (2014).
Messenger RNA degradation in bacterial cells.
Annu. Rev. Genet. 48: 537-559.
Hsieh PK, Richards J, Liu Q, Belasco JG (2013).
Specificity of RppH-dependent RNA degradation in Bacillus subtilis.
Proc. Natl. Acad. Sci. USA 110: 8864-8869.
Lodato PB, Hsieh PK, Belasco JG, Kaper JB (2012).
The ribosome binding site of a mini-ORF protects a T3SS mRNA from degradation by RNase E.
Mol. Microbiol. 86: 1167-1182.
Richards J, Luciano DJ, Belasco JG (2012).
Influence of translation on RppH-dependent mRNA degradation in Escherichia coli.
Mol. Microbiol. 86: 1063-1072.
Luciano DJ, Hui MP, Deana A, Foley PL, Belasco KJ, Belasco JG (2012).
Differential control of the rate of 5′-end-dependent mRNA degradation in Escherichia coli.
J. Bacteriol. 194: 6233-6239.
Yao S, Richards J, Belasco JG, Bechhofer DH (2011).
Decay of a model mRNA in Bacillus subtilis by a combination of RNase J1 5′ exonuclease and RNase Y endonuclease activities.
J. Bacteriol. 193: 6384-6386.
Richards J, Liu Q, Pellegrini O, Celesnik H, Yao S, Bechhofer DH, Condon C, Belasco JG (2011).
An RNA pyrophosphohydrolase triggers 5′-exonucleolytic degradation of mRNA in Bacillus subtilis.
Mol. Cell 43: 940-949.
Richards J, Belasco JG (2011).
Ribonuclease J: how to lead a double life.
Structure 19: 1201-1203.
Belasco JG (2010).
All things must pass: contrasts and commonalities in eukaryotic and bacterial mRNA decay.
Nature Rev. Mol. Cell Biol. 11: 467-478.
Savas JN, Ma B, Deinhardt K, Culver BP, Restituito S, Wu L, Belasco JG, Chao MV, Tanese N (2010).
A role for Huntington’s disease protein in dendritic neuronal RNA granules.
J. Biol. Chem. 285: 13142-13153.
Piao X, Zhang X, Wu L, Belasco JG (2010).
CCR4-NOT deadenylates mRNA associated with RNA-induced silencing complexes in human cells.
Mol. Cell. Biol. 30: 1486-1494.
Schuck A, Diwa A, Belasco JG (2009).
RNase E autoregulates its synthesis in Escherichia coli by binding directly to a stem-loop in the rne 5′ untranslated region.
Mol. Microbiol. 72: 470-478.
Messing SAJ, Gabelli SB, Liu Q, Celesnik H, Belasco JG, Piñeiro SA, Amzel M (2009).
Structure and biological function of the RNA pyrophosphohydrolase BdRppH from Bdellovibrio bacteriovorus.
Structure 17: 472-481.
Wu L, Belasco JG (2008).
Examining the influence of microRNAs on translation efficiency and on mRNA deadenylation and decay.
Methods Enzymol. 449: 373-393.
Celesnik H, Deana A, Belasco JG (2008).
PABLO analysis of RNA: 5′-phosphorylation state and 5′-end mapping.
Methods Enzymol. 447: 83-98.
Richards J, Belasco JG (2008).
A new window onto translational repression by bacterial sRNAs.
Mol. Cell 32: 751-753.
Wu L, Fan J, Belasco JG (2008).
Importance of translation and nonnucleolytic Ago proteins for on-target RNA interference.
Curr. Biol. 18: 1327-1332.
Wu L, Belasco JG (2008).
Let me count the ways: mechanisms of gene regulation by miRNAs and siRNAs.
Mol. Cell 29: 1-7.
Deana A, Celesnik H, Belasco JG (2008).
The bacterial enzyme RppH triggers messenger RNA degradation by 5′ pyrophosphate removal.
Nature 451: 355-358.
Celesnik H, Deana A, Belasco JG (2007).
Initiation of RNA decay in Escherichia coli by 5′ pyrophosphate removal.
Mol. Cell 27: 79-90.
Wu L, Fan J, Belasco JG (2006).
MicroRNAs direct rapid deadenylation of mRNA.
Proc. Natl. Acad. Sci. USA 103: 4034-4039.
Deana A, Belasco JG (2005).
Lost in translation: the influence of ribosomes on bacterial mRNA decay.
Genes Dev. 19: 2526-2533.
Wu L, Belasco JG (2005).
Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells.
Mol. Cell. Biol. 25: 9198-9208.
Jiang X, Belasco JG (2004).
Catalytic activation of multimeric RNase E and RNase G by 5′-monophosphorylated RNA.
Proc. Natl. Acad. Sci. USA 101: 9211-9216.
Deana A, Belasco JG (2004).
The function of RNase G in Escherichia coli is constrained by its amino and carboxyl termini.
Mol. Microbiol. 51: 1205-1217.
Diwa AA, Jiang X, Schapira M, Belasco JG (2002).
Two distinct regions on the surface of an RNA-binding domain are crucial for RNase E function.
Mol. Microbiol. 46: 959-969.
Belasco JG (2002).
Use of T7 phage display to clone RNA-binding protein cDNAs.
inNovations 15: 7-9.
Diwa AA, Belasco JG (2002).
Critical features of a conserved RNA stem-loop important for feedback regulation of RNase E synthesis.
J. Biol. Chem. 277: 20415-20422.
Feldbrügge M, Arizti P, Sullivan ML, Zamore PD, Belasco JG, Green PJ (2002).
Comparative analysis of the plant mRNA-destabilizing element, DST, in mammalian and tobacco cells.
Plant Mol. Biol. 49: 215-223.
Jain C, Deana A, Belasco JG (2002).
Consequences of RNase E scarcity in Escherichia coli.
Mol. Microbiol. 43: 1053-1064.
Danner S, Belasco JG (2001).
T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries.
Proc. Natl. Acad. Sci. USA 98: 12954-12959.
Jain C, Belasco JG (2001).
Structural model for the cooperative assembly of HIV-1 Rev multimers on the RRE as deduced from analysis of assembly-defective mutants.
Mol. Cell 7: 603-614.
Jain C, Belasco JG (2000).
Rapid genetic analysis of RNA-protein interactions by translational repression in Escherichia coli.
Methods Enzymol. 318: 309-332.
Diwa A, Bricker AL, Jain C, Belasco JG (2000).
An evolutionarily conserved RNA stem-loop functions as a sensor that directs feedback regulation of RNase E gene expression.
Genes Dev. 14: 1249-1260.
Jiang X, Diwa A, Belasco JG (2000).
Regions of RNase E important for 5′-end-dependent RNA cleavage and autoregulated synthesis.
J. Bacteriol. 182: 2468-2475.
Bricker AL, Belasco JG (1999).
Importance of a 5′ stem-loop for longevity of papA mRNA in Escherichia coli.
J. Bacteriol. 181: 3587-3590.
Rimmele ME, Belasco JG (1998).
Target discrimination by RNA-binding proteins: role of the ancillary protein U2A' and a critical leucine residue in differentiating the RNA-binding specificity of spliceosomal proteins U1A and U2B".
RNA 4: 1386-1396.
Laird-Offringa IA, Belasco JG (1998).
RNA-binding proteins tamed.
Nature Struct. Biol. 5: 665-668.
Arnold TE, Yu J, Belasco JG (1998).
mRNA stabilization by the ompA 5′ untranslated region: two protective elements hinder distinct pathways for mRNA degradation.
RNA 4: 319-330.
Jain C, Belasco JG (1997).
A rapid genetic method for the study of RNA binding proteins.
In: Richter JD, ed. mRNA Formation and Function. Academic Press. 263-284.
Bouvet P, Jain C, Belasco JG, Amalric F, Erard M (1997).
RNA recognition by the joint action of two nucleolin RNA-binding domains: genetic analysis and structural modeling.
EMBO J. 16: 5235-5246.
Jain C, Belasco JG (1996).
A structural model for the HIV-1 Rev-RRE complex deduced from altered-specificity Rev variants isolated by a rapid genetic strategy.
Cell 87: 115-125.
Aristarkhov A, Mikulskis A, Belasco JG, Lin ECC (1996).
Translation of the adhE transcript to produce ethanol dehydrogenase requires RNase III cleavage in Escherichia coli.
J. Bacteriol. 178: 4327-4332.
Wickens M, Belasco JG, Jacobson A (1996).
Changes in the length of poly(A) tails and their effects on mRNA translation and turnover.
In: Resnekov O and von Gabain A, eds. Post-transcriptional Control of Gene Expression. Springer-Verlag. 45-55.
Belasco JG (1996).
Mechanisms and structural determinants of mRNA decay in bacteria.
In: Resnekov O and von Gabain A, eds. Post-transcriptional Control of Gene Expression. Springer-Verlag. 37-43.
Laird-Offringa IA, Belasco JG (1996).
In vitro genetic analysis of RNA-binding proteins using phage display libraries.
Meth. Enzymol. 267: 149-168.
Laird-Offringa IA, Belasco JG (1995).
Analysis of RNA-binding proteins by in vitro genetic selection: identification of an amino acid residue important for locking U1A onto its RNA target.
Proc. Natl. Acad. Sci. USA 92: 11859-11863.
Jain C, Belasco JG (1995).
Autoregulation of RNase E synthesis in Escherichia coli.
Nucleic Acids Symp. Ser. 114: 85-88.
Zubiaga AM, Belasco JG, Greenberg ME (1995).
The nonamer UUAUUUAUU is the key AU-rich sequence motif that mediates mRNA degradation.
Mol. Cell. Biol. 15: 2219-2230.
Jain C, Belasco JG (1995).
RNase E autoregulates its synthesis by controlling the degradation rate of its own mRNA in Escherichia coli: unusual sensitivity of the rne transcript to RNase E activity.
Genes Dev. 9: 84-96.
Hansen MJ, Chen LH, Fejzo MLS, Belasco JG (1994).
The ompA 5′ untranslated region impedes a major pathway for mRNA degradation in Escherichia coli.
Mol. Microbiol. 12: 707-716.
Schiavi SC, Wellington CL, Shyu AB, Chen CY, Greenberg ME, Belasco JG (1994).
Multiple elements in the c-fos protein-coding region facilitate mRNA deadenylation and decay by a mechanism coupled to translation.
J. Biol. Chem. 269: 3441-3448.
Greenberg ME, Belasco JG (1993).
Control of the decay of labile protooncogene and cytokine mRNAs.
In: Belasco JG and Brawerman G, eds. Control of Messenger RNA Stability. Academic Press. 199-218.
Belasco JG, Brawerman G (1993).
Experimental approaches to the study of mRNA decay.
In: Belasco JG and Brawerman G, eds. Control of Messenger RNA Stability. Academic Press. 475-493.
Belasco JG (1993).
mRNA degradation in prokaryotic cells: an overview.
In: Belasco JG and Brawerman G, eds. Control of Messenger RNA Stability. Academic Press. 3-12.
Wellington CL, Greenberg ME, Belasco JG (1993).
The destabilizing elements in the coding region of c-fos mRNA are recognized as RNA.
Mol. Cell. Biol. 13: 5034-5042.
Wellington CL, Belasco JG (1992).
Autoregulation through translation.
Current Biol. 2: 216-218.
Schiavi SC, Belasco JG, Greenberg ME (1992).
Regulation of proto-oncogene mRNA stability.
Biochim. Biophys. Acta 1114: 95-106.
Bouvet P, Belasco JG (1992).
Control of RNase E-mediated RNA degradation by 5′-terminal base pairing in E. coli.
Nature 360: 488-491.
Emory SA, Bouvet P, Belasco JG (1992).
A 5′-terminal stem-loop structure can stabilize mRNA in Escherichia coli.
Genes Dev. 6: 135-148.
Chen LH, Emory SA, Bricker AL, Bouvet P, Belasco JG (1991).
Structure and function of a bacterial mRNA stabilizer: analysis of the 5′ untranslated region of ompA mRNA.
J. Bacteriol. 173: 4578-4586.
Shyu AB, Belasco JG, Greenberg ME (1991).
Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.
Genes Dev. 5: 221-231.
Greenberg ME, Shyu AB, Belasco JG (1990).
Deadenylylation: a mechanism controlling c-fos mRNA decay.
Enzyme 44: 181-192.
Belasco JG, Shyu AB, Greenberg ME (1990).
Rapid degradation of the c-fos proto-oncogene transcript.
In: McCarthy JEG and Tuite MF, eds. Post-transcriptional Control of Gene Expression. Springer-Verlag. 65-71.
Chen CYA, Belasco JG (1990).
Degradation of pufLMX mRNA in Rhodobacter capsulatus is initiated by non-random endonucleolytic cleavage.
J. Bacteriol. 172: 4578-4586.
Emory SA, Belasco JG (1990).
The ompA 5′ untranslated RNA leader functions in Escherichia coli as a growth-rate regulated mRNA stabilizer whose activity is unrelated to translational efficiency.
J. Bacteriol. 172: 4472-4481.
Shyu AB, Greenberg ME, Belasco JG (1989).
The c-fos transcript is targeted for rapid decay by two distinct mRNA degradation pathways.
Genes Dev. 3: 60-72.
Belasco JG, Chen CYA (1988).
Mechanism of puf mRNA degradation: the role of an intercistronic stem-loop structure.
Gene 72: 109-117.
Belasco JG, Higgins CF (1988).
Mechanisms of mRNA decay in bacteria: a perspective.
Gene 72: 15-24.
Chen CYA, Beatty JT, Cohen SN, Belasco JG (1988).
An intercistronic stem-loop structure functions as an mRNA decay terminator necessary but insufficient for puf mRNA stability.
Cell 52: 609-619.
Nilsson G, Belasco JG, Cohen SN, von Gabain A (1987).
Effect of premature termination of translation on mRNA stability depends on the site of ribosome release.
Proc. Natl. Acad. Sci. USA 84: 4890-4894.
Klug G, Adams CW, Belasco J, Doerge B, Cohen SN (1987).
Biological consequences of segmental alterations in mRNA stability: effects of deletion of the intercistronic hairpin loop region of the R. capsulatus puf operon.
EMBO J. 6: 3515-3520.
Fisher LM, Belasco JG, Bruice TW, Albery WJ, Knowles JR (1986).
Proline racemase: oversaturation and interconversion of enzyme forms.
In: Frey PA, ed. Proceedings of the 15th Steenbock Symposium. Elsevier. 205-216.
Belasco JG, Bruice TW, Fisher LM, Albery WJ, Knowles JR (1986).
Energetics of proline racemase: rates, fractionation factors, and buffer catalysis in the oversaturated region. Nature of the interconversion of the two forms of free enzyme.
Biochemistry 25: 2564-2571.
Belasco JG, Bruice TW, Albery WJ, Knowles JR (1986).
Energetics of proline racemase: fractionation factors for the essential catalytic groups in the enzyme-substrate complexes.
Biochemistry 25: 2558-2564.
Belasco JG, Albery WJ, Knowles JR (1986).
Energetics of proline racemase: double fractionation experiment, a test for concertedness and for transition-state dominance.
Biochemistry 25: 2552-2558.
Fisher LM, Belasco JG, Bruice TW, Albery WJ, Knowles JR (1986).
Energetics of proline racemase: transition-state fractionation factors for the two protons involved in the catalytic steps.
Biochemistry 25: 2543-2551.
Belasco JG, Nilsson G, von Gabain A, Cohen SN (1986).
The stability of E. coli gene transcripts is dependent on determinants localized to specific mRNA segments.
Cell 46: 245-251.
Belasco JG, Beatty JT, Adams CW, von Gabain A, Cohen SN (1985).
Differential expression of photosynthesis genes in R. capsulata results from segmental differences in stability within the polycistronic rxcA transcript.
Cell 40: 171-181.
Nilsson G, Belasco JG, von Gabain A, Cohen SN (1984).
Growth-rate dependent regulation of mRNA stability in Escherichia coli.
Nature 312: 75-77.
von Gabain A, Belasco JG, Schottel JL, Chang, ACY, Cohen SN (1983).
Decay of mRNA in Escherichia coli: investigation of the fate of specific segments of transcripts.
Proc. Natl. Acad. Sci. USA 80: 653-657.
Belasco JG, Albery WJ, Knowles JR (1983).
Double isotope fractionation: test for concertedness and for transition state dominance.
J. Am. Chem. Soc. 105: 2475-2477.
Belasco JG, Knowles JR (1983).
Polarization of substrate carbonyl groups by yeast aldolase: investigation by Fourier transform infrared spectroscopy.
Biochemistry 22: 122-129.
Fisher J, Belasco JG, Charnas RL, Khosla S, Knowles JR (1983).
Beta-lactamase inactivation by mechanism based reagents.
Phil. Trans. R. Soc. Lond. B. 289: 309-319.
Fisher J, Belasco JG, Khosla S, Knowles JR (1980).
Beta-lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin.
Biochemistry 19: 2895-2901.
Belasco JG, Knowles JR (1980).
Direct observation of substrate distortion by triosephosphate isomerase using Fourier transform infrared spectroscopy.
Biochemistry 19: 472-477.
Belasco JG, Herlihy JM, Knowles JR (1978).
Critical ionization states in the reaction catalyzed by triosephosphate isomerase.
Biochemistry 17: 2971-2978.
Richards J, Belasco JG (2023).
Graded impact of obstacle size on scanning by RNase E.
Nucleic Acids Res. 51: 1364-1374.